Reporter
RUBY

Part:BBa_K4201012

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


RUBY

Chimeric expression of RUBY reporter in G. max allows visualization of an genomically integrated construct.

RUBY is a reporter construct that is composed of the enzymes that are needed for the biosynthesis of betalain1. Betalain is a naturally occurring compound found in Caryophyllales and it produces a bright red color2. When used as a reporter, it will turn the transgenic plant tissue red and it does not need UV light to observe like a GFP reporter would, as seen in the figure below. The RUBY reporter allows for the survival of the modified plant unlike a GUS reporter, which is lethal to the plant.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3745
    Illegal BamHI site found at 2394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 330
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 2622
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 3968


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Categories
Parameters
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